Abstract: Conflicts between ecosystem conservation and urbanization has threatened the human well-being. This study evaluates both supply and demand of water yield, carbon sequestration, and food production in the Hang-Jia-Hu region in 2000, 2005 and 2010 from perspective of “Water-Energy-Food” nexus. Spatial Gini-coefficient model, supply-demand ratio model and correlation model are applied to analysis spatial heterogeneities. Results show: (1) The yielding water decreased first and then rebounded, while the total amount of carbon sequestration and food production decreased slightly. The demand of water and food increased slightly, while the demand of carbon sequestration increased sharply. (2) The supply of water and carbon sequestration is significant within the hilly area located in the southwest. While the food production is clustered in the eastern plain. The demand of all three ecosystem services is inevitably concentrated in the densely populated areas. (3) The spatial distribution of evaluated ecosystem services is basically balanced, but the mismatch between supply and demand is prominent. Most areas suffered shortage of water, and urban area had hard pressure of carbon sequestration. (4) Water supply has a “trade-off” relation with carbon sequestration supply, while a “synergy” relation with food supply. Carbon sequestration supply is positive with food supply. Demand of all the three ecosystem services is positive with each other. WEF nexus is proved to show an interactive and supportive relationship in the region. Several adaptive polices are promoted to improve the regional ecosystem.