Abstract: Based on the fish resources surveys in Jianli section of the Yangtze River from 2013 to 2015 and 2017 to 2018, the fish community structure and species diversity were described using ShannonWiener diversity index, Simpson dominance index, Margalef’s richness index, Pielou’s evenness index and multivariate statistical analysis, respectively. A total of 68 fish species belonging to 13 families and 6 orders were collected during monitoring periods. They were dominated by Cypriniforme. The numbers of fish species in 2018 were more than those in other years. There were no significant variations in Pielou’s evenness index in different years, while ShannonWiener diversity index and Margalef’s richness index in 2018 were highest in all years. Simpson dominance index fluctuated to some extent, but they were all low in the five years. The abundancebiomass curves (ABC) and Wstatistical analysis indicated that fish community was suffering with high disturbance in 2013 and 2014, while the community was not disturbed in 2015, 2017 and 2018. The fish community structures in the five years were divided into three groups by using cluster analysis and nonmetric multidimensional scaling (NMDS) methods．No significant difference was found by the analysis of similarity statistics (ANOSIM)  during these three groups.