Abstract: In order to clarify the trade-off / synergy relationship between the main ecosystem services of typical karst mountain area, taking Wenshan city of Yunnan Province as the research object, based on theoretical framework of Millennium Ecosystem Assessment (MA), four ecosystem services including food supply, net primary productivity (NPP), soil conservation and water yield were selected to evaluate the temporal and spatial changes of land use and ecosystem services with the application of CASA model, InVEST model, RUSLE model and related analysis methods. The results show that from 2000 to 2017, the area of forest and unused land in Wenshan City has decreased, and other land use types have increased relatively, of which construction land has increased the most; The annual average value of ecosystem service in Wenshan City has shown an overall increasing trend in 17 years, with inter-annual changes increasing and decreasing; There is a synergistic relationship between water yield and food supply, both of them are in a trade-off relationship with NPP and soil conservation, and there is a synergistic relationship between NPP and soil conservation; In terms of spatial distribution, the ecosystem services are mainly synergistic, the spatial patterns of trade-off / synergy relationship are different, which show that Wenshan downtown area has significant synergy or significant trade-off relationship, while other areas in the study area are scattered.