Abstract: Jingjiang section of Yangtze River is host to plenty of migratory fishes. For the sake of grasping the characteristics of food web structure in the nearshore zone of Jingjiang section, together with providing basic biological data for further study and fishery resource assessment, this study employed stable isotope technique of carbon and nitrogen to detect the δ13C and δ15N values of each biological sample collected there in August 2016 and constructed the continuous trophic spectrum of crabs, small fishes, shrimps and other fishery creatures. As the results revealed, the δ13C value of zooplankton was -30.34‰ and the lowest, followed by the benthic clastic of -30.24‰. The average δ13C values of shellfish, aquatic plants, small fishes and shrimps were -29.75‰±0.75‰, -28.98‰±0.88‰, -26.74‰±2.37‰ and -26.1‰±0.16‰, respectively. Crabs owned the highest δ13C value of -25.86‰±0.74‰. The δ15N value of aquatic plants was 5.16‰±0.85‰ and the lowest, and benthic clastic of 5.58‰ in the next place. The average δ15N values of shellfish, small fishes, zooplankton and crabs were 8.13‰±0.31‰, 11.56‰±1.59‰, 12.02‰ and 12.51‰±1.43‰, respectively. Shrimps possessed the highest δ15N value of 13.05‰±0.45‰. The order of trophic level was aquatic plants, benthic clastic, shellfish, zooplankton, small fish, crabs and shrimps from low to high. Small fishes, crabs and shrimps in this part of water area shared the similar food source while the stable isotope values suggested an obvious overlap in trophic niche among them.