Abstract: The Three Gorges Reservoir area of Chongqing, located on the upper area of the Yangtze River Basin, is one of the most important biodiversity conservation sites of China. It possesses abundant species and land resources. However, various human activities have directly or indirectly influenced the ecosystem pattern, and consequently the landscape pattern of Three Gorges Reservoir area has significantly changed. Therefore, based on the ArcGIS and FRAGSTATS software, the landscape type map in the Three Gorges Reservoir Area of Chongqing influenced by human activities was obtained through the method of artificial interpretation with the 3 remote sensing images (1995,2005,2015). DMSP/OLS nighttime light stable data was selected to reflect the intensity of human activities, which was divided into 10 levels from weak to strong using the natural breaks method. Besides, the change characteristics of the landscape pattern in different periods within different transects were analyzes, as well as the gradient response of 4 types of landscape pattern characteristics to human activity intensity in 2015. The results were as follows:1) The spatial distribution of human activities was significantly different, which gradually decreases from west to east. In addition, the intensity of human activities in the Yangtze River Basin was obviously stronger than that in the surrounding area.2) At the landscape level, as the human activity intensity increased, the number of landscape patches in the study area was greatly reduced; yet SHDI (Shannon diversity index) and SHEI (Shannon evenness index) first ascended and then descended. In addition, landscape connectivity decreased slightly, and landscape aggregation increased, as well as the fragmentation degree while the situation improved in 2015.3) At the class level, the change of landscape pattern could be well reflected by the 4 main landscape types (construction land, water area, grass land and low density vegetation cover forest land). Especially, PD (patch density), LSI (landscape shape index) and MPFD (mean patch fractal dimension) had significant variations with the gradient of human activity intensity, LPI (largest patch index) and PLAND (percentage of landscape) had a small change except construction land, which indicated that landscape shape was becoming increasingly simplified and regular. PLAND and LPI of construction land reached to 71.2% and 35.72% respectively when human interfered to the most extent. 4) Three Gorges project, the resettlement project, urbanization and the grain for green policy were essential driving forces for the evolution of landscape pattern in the Three Gorges Reservoir area of Chongqing. This study revealed the gradient change of landscape pattern along the intensity of human activities in the Three Gorges Reservoir area of Chongqing in recent 20 years, which could provide references for the rational use of natural resources and sustainable development in the Three Gorges Reservoir area of Chongqing.