Abstract: In order to provide basis for the regulation of closed fishing seasons and areas, and for protection of germplasm resources, spatial and temporal distribution characteristics of spawning eggs, larvae and juveniles were studied in the Aquatic Germplasm National Reserve of Corbiculafluminea Culter alburnus, Dianshan Lake, from May to August 2016. The results showed that 4463 fish eggs were collected totally, 43012 larvae and juveniles were identified, belonging to 6 orders, 9 families, 19 genuses and 21 species. In addition, the number of individuals caught in fish eggs and in larval fish was approximately 4.16% and 3.98%, respectively. Interestingly, the spawning starts on June 13 while capture is banned on August 17. The relative proportion of the C. alburnus peaked in the mid late July and early August. During the survey, the spawning water temperature was above 25.9℃, and increased to 29.3-34.3℃ during the spawning peak. It is prone that the C. alburnus spawn in clear nights just after rain when the water level rises, and the fish begin to spawn actively when the water level is up to 2 m and other conditions are appropriate. The result also showed that the spawning density of the C. alburnus has a close relation to water temperature and water level (P<0.01). According to the results of the early resources, the collected eggs in artificial fish nests, and the sampled brood brooders in ovulation period, it was concluded that No. 1（Qian deng pu qiao), No. 2(Mei bang), and No. 7(Nan shen bang) waters were the spawning grounds for the C. alburnus.