Abstract: The evaluation of ecological service value is a kind of economic quantitative assessment of ecology function. This paper employed various methods including replacement engineering method, shadow engineering method, carbon tax law, market value method and water balance method to evaluate the ecological services value of the natural resources in the Three Gorges Reservoir (TGR) based on the net primary productivity dataset (NPP), land use/land cover change (LUCC), Remote Sensing data, climate data and statistical data in 2000, 2005, 2010 and 2015, with GIS technology, respectively. And analyze of changes in its temporal and spatial patterns. The results show, from 2000 to 2015, the total value of ecological services in this area increased by 26.937 billion yuan, with a growth rate of 15.32%. The most contribution to total value was made by forest ecosystem with a proportion of 48.14%, followed by cropland with 44.04%. The changing value of forest ecosystem services is mainly correlated with expanding forest area. The area of cropland decreased by 4.64%, whereas the total services value of cropland increased by 14.54%, which means that croplands in this region were used unreasonably. As for grassland, the area decreased by 16.57% along with 5.01% decrease of services value, for which protection of grassland ecosystems should be strengthened. Overall, the implementation of the TGR has a greater impact on the ecosystem services than the disadvantages on the reservoir area globally. Reasonable and efficient land use plan and environment protection plan should be made to ensure the security of the TGR, sustainable economic, and ecological development in the future.