Abstract: In order to know the annual dynamics and migration characteristics of fish resources in the channel connecting Dongting Lake and the Yangtze River, A monthly surveys were conducted for larval, juvenile and adult fishes in the channel connecting Dongting Lake and the Yangtze River from May 2017 to June 2018. Larvae fishes were belonging to 19 species and 5 families. Hemiculter leucisculus (Basilewsky) accounted for 76.6% of the total, Rhinogobius giurinus (Rutter) accounted for 8,8% of the total, Pseudobrama simoni (Bleeker) accounted for 4.3% of the total. Larva appeared mainly in Apr to Sep, and the density was highest in June (848.8 Ind./1 000 m3). Also juvenile and adult fishes were belonging to 42 species and 9 families. Coilia brachygnathus (Kreyenberg et Pappenheim) accounted for 30.3% of the total, Hemiculter bleekeri (Warpachowsky) accounted for 15.3% of the total, and Hemiculter leucisculus (Basilewsky) accounted for 12.2% of the total. The abundance of juvenile and adult fishes was 729 each day also was the highest in Aug. The results showed that the abundance of larvae, juvenile and adult fishes showed strongly seasonal variation, both of them were higher in summer and lower in winter. From April to July, the movement index of the river-lake migratory fishes was between 0.1 and 0.7, showing a tendency of entering the lake. From August to march, the movement index was between -0.4and -0.1, showing a tendency of entering the river. The results also showed that juvenile and adult fish migrated mainly at night. And discharge were the main driving factors of fish.