Abstract: To explore the community structure of metazooplankton (referred to as zooplankton) and the status of water quality in Jinshuitan reservoir, the zooplankton community and water quality parameters were investigated and analyzed from October 2019 (autumn), January 2020 (winter), May 2020 (spring), and August 2020 (summer). Totally 33 species of zooplankton distributed in 21 genera and 3 phyla were identified. Among them, the species of cladocera were the most (13 species), followed by rotifer (11 species) and copepods (9 species), Cladocera and ritifer were the dominant zooplankton.. The abundance of zooplankton was the highest in summer (311.7 ind.·L^-1) and the lowest in winter (135.5 ind.·L^-1). Cladocera and copepods were more abundance. Moreover, the result of cluster analysis indicated that the zooplankton structures from spring and winter were similar, and zooplankton structures from summer and autumn were similar. Only three species, Thermocyclops vermifer, Bosmina longirostris, and B. coregoni, were dominant, demonstrating low dominance and simple structure of zooplankton community in the reservoir. The diversity indices (1.75<H′<2.64, 0.71<J<0.90, and 1.93<D<3.80) showed that the pollution levels of water quality in the reservoir is slight or intermediate. The results of redundancy analysis (RDA) indicated that water temperature (WT), water depth (WD), total nitrogen (TN), pH, total phosphorus (TP) and dissolved oxygen (DO) were main factors affecting zooplankton community structure, while transparency (SD) and permanganate index (CODMn) also have effects on the zooplankton community structure.