Abstract: Various waterbodies including ditches, ponds, and paddy fields are important components of  polders wetlands. Maintaining reasonable concentrations of key biogenic elements including carbon, nitrogen and phosphorus in polders is essential for the ecological function of wetlands. Taking the polders along the Le 'an River in Poyang Lake Basin as an example, this study explored the distribution pattern of concentrations of dissolved carbon (DOC), nitrogen (DTN), and phosphorus (DTP) in major waterbodies of polders, through field monitoring and sampling. A quantitative study was also conducted to reveal the effect of hydrological connectivity on element concentrations. All elements were distributed in the hierarchy of paddy field > pond > ditch, with mean concentrations of 9.5/13.8/19.0/34.2 mg/L (DOC)，0.6/1.1/0.82/8.8 mg/L (DTN)，0.016/0.025/0.036/0.12 mg/L(DTP), respectively. Concentration of biogenic elements was strongly correlated with hydrological connectivity, and the latter could explain up to 25.4% of the change of the concentrations. This finding implied that an improvement of connectivity could shorten the residence time of the elements and accelerate the turnover rates, and consequently resulted in decreased concentrations. The demand of flood control and water supply requires the necessary to control the concentrations of biogenic elements in polders. Therefore, in practice, it is suggested to manage the timing and duration of sluice gate opening/closing in order to restore an improved hydrological connectivity in polders.