Abstract: Comparing the characteristics of industrial spatial organization of mature and growing metropolitan areas and identifying their formation mechanisms can help summarize the universal laws of industrial spatial organization in metropolitan areas, and provide a basis for optimizing industrial spatial organization in growing metropolitan areas. Taking the Shanghai metropolitan area and the Wuhan metropolitan area as examples, this study adopted the logic of comparative study to identify the common patterns and differences of industrial spatial organization in spatial agglomeration, functional collaboration and formation mechanisms of the two metropolitan areas. The results showed that in terms of spatial agglomeration characteristics, the Shanghai metropolitan area presented a gradient configuration of industrial chain division pattern; The Wuhan metropolitan area presented a near-regional organization, with a strong manufacturing links, a weak R&D and service link, and links in the industry chain were synergistically clustered in the near-region circle; It was found that a distance of 10 km was the key distance node for the combination of production and innovation factors of the two metropolitan areas, but the outer circle of Wuhan metropolitan area lacked the support of innovation factors. In terms of functional collaboration characteristics, the Shanghai metropolitan area were much stronger than the Wuhan metropolitan area in terms of network capacity, density and cooperation efficiency. The Shanghai metropolitan area was a polycentric network with both neighboring organizations and leapfrog organizations t, while the Wuhan metropolitan area was a single-center network with neighboring organizations; The near-circle organization of supply chain was a common feature of the two metropolitan areas, but the supplier self-sufficiency rate of the Wuhan metropolitan area was low. Finally, based on the comparative results and mechanism analysis of the characteristics of industrial spatial organization, this study put forward corresponding suggestions for the optimization organization of industrial space in growing metropolitan area from the perspective of core strengthening, functional collaboration innovation-driven and strengthening the sub-nucleus.