Abstract: For accurately predicting foliar uptake of the Naphthalene (Nap), Phenanthrene (Phe), Fluorene (Fl) and Pyrene (Pyr), leaf cuticle of Pinus massoniana，Pittosporum. Tobira and Osmanthus fragrans were isolated by chemical methods. Adsorption behavior of PAHs onto leaf cuticle was investigated and the their influence factors. The three tree species cuticle were obtained by means of the chemical separation. The results showed that the distribution of PAHs was mainly responsive for the absorption of Nap, Phe, Fl and Pyr. According to the value of Kd, which was the by the rate of linear isothermal adsorption curve, three tree species had a strong sorption capacity of Nap. P. tobira had a stronger sorption capacity than O. fragrans and P. tobira. P. massoniana had an similar adsorption capacity of Phe compared with O. fragrans, but stronger than that of P. tobira. P. massoniana had a stronger sorption capacity of Fl than O. fragrans and P. tobira. O. fragrans had a stronger sorption capacity of Pyr than P. massoniana and P. tobira. An scanning electron microscope (SEM) observation revealed that the P. sylvestris cuticle has a rich microstructure, with stoma distributed densely on both sides. The surface of O. fragrans cuticle had rough folds, whereas P. tobira cuticle was smooth. However, we did not observe an obvious stoma in both P. tobira and O. fragrans cuticle. The differences in the leaf microstructure of plant cuticle were major causes of the interspecies difference of PAHs (polycyclic aromatic hydrocarbons, PAHs) adsorption. A Fourier transform infrared (FTIR) analysis revealed that P. massoniana contained fatty components, with the highest lipid content among the three trees. We also observed signals of three plant cuticle in C=O (~1 734 cm^-1), indicating an obvious absorption peak of ester bond. Moreover, C-O-C peak (~1 057 cm^-1) is most prominent, which related to sugar content.