Abstract: The lack of market trust in the producer will lead to the serious shortage of green agricultural products supply and lead to the structural imbalance of food supply side. Based on 804 survey data of rice growers in Hubei province, the influences of market trust and institutional environment on the application behavior of biological pesticides of farmers were discussed. The results show：（1）Farmers not only lack trust in the consumption of agricultural products sold on the market, but also lack trust in the production of green agricultural products. 67.79% of the sample farmers are worried about the market sales of green agricultural products. （2）Market trust can significantly promote farmers’ application of biological pesticides. Compared with incentive, constraint system has a greater impact on farmers’ behavior, and market incentive and constraint system can effectively supplement farmers’ lack of market trust. （3）Governments and enterprises have different influences on farmers’ application of biological pesticides. Both enterprises and governments can significantly promote farmers’ application, but the effect of enterprises’ constraint on farmers’ green production is better than government. Therefore, it is extremely necessary to enhance farmers’ trust in the green agricultural product market, strengthen the construction of market institutional environment, and advocate multi-governance to make up for the limitation of government functions.