Abstract: The study of urbanization's role in extreme temperature events can help to understand the evolution of these events and to reduce their damage. Based on daily meteorological observation data and land use/ cover change data from urban and rural sites in urban agglomeration in the middle reaches of the Yangtze River during 2000~2020, this study analyzed the temporal trends of extreme temperature events. Sen's slope estimation and the Mann-Kendall significance test were adopted in this study. The impact of urbanization and changes in landscape patterns on extreme temperature events was also discussed, in terms of the contribution rate. The results showed: (1) From 2000 to 2020, the overall trend of extreme temperature events at urban and rural sites in the study area was in the same direction, with an increasing trend in extreme high temperatures and a decreasing trend in extreme low temperatures. (2) Urbanization intensified the frequency of extreme temperature events at night. Impact of urbanization on the number of hot nights (TR) and cold nights (TN10p) was calculated as 0.19 days per decade and 0.27 days per decade, respectively, with the corresponding contribution rates of 49.79% and 74.08%. (3) The increase in the proportion of construction land patch area, patch density, and total edge length, as well as the overall increase in landscape fragmentation, diversity, and shape complexity, demonstrated the strongest correlation with the number of hot nights (TR) and the minimum extreme low temperature (TNn). The total edge length of construction land (TE_C) had the largest correlation coefficient with TR (0.25). Overall, urbanization noticeably affected the frequency and intensity of extreme temperature events in urban agglomeration in the middle reaches of the Yangtze River.