Abstract: The ecological environment is the essential requirement for living of human beings. In the recent years, the extensive mode of development at the expense of the environment leaded to the ecological environment worsening and seriously harmed the sustainable development and the countrys ecological security. Under these circumstances, the country put forward the ecological red-line, which is an insurmountable bottom line. With the aim to keep the balance of ecology and the need of sustainable development, to draw the ecological red-line of fragile and important ecological zone is the priority of environmental protection in China. Guizhou Chishui River Basin has its special geographical environment and is the production place of famous white spirit "Maotai". It has also national nature reserves. It plays an important role in special water resources, soil conservation and species diversity. This study was carried out based on the ecological red-line analysis of theory and methodology and chose the Chishui River as the study case to evaluate the sensitivity of karst rocky desertification and soil erosion, the importance of soil conservation, water conservation, and species diversity, and to find the most sensitive ecological zone and most important ecological function zone. An overlay analysis of development prohibition zone of spirit production in Chishui River and water conservation zone of Maotai was also carried out and the boundary of ecological red-line was determined in the end. The major results are as followed:There are 9 kinds of ecological red-line, being 5 030.58 km² in area, accounting for 44.16% of the total. There are many overlapping red-line zones in Chishui City and the important ecological function zone represents a significant share due to the special features of geography. The red line areas are mainly distributed in national nature reserve of Chishui City and Xishui County. Furthermore, with the consideration of local ecological environment and human activities in the comprehensive characteristics and the objective demand of the development, we also offer some suggestions for the management of ecological red-line zones in the Chishui River. First, adhere to the principle of the priority of special water resource conservation and pay equal attention to ecological protection and construction, combined with classification management and integrated management. Second, restrict access of industrial development and establish the mechanism of ecological compensation. Third, arrange reasonabl land use in red-line zones, carry out agricultural production without affecting the quality of ecological environment, and build a friendly way of land use. Forth, take the path of sustainable development to realize healthy and coordinated development of regional economic and environmental.

Key words: ecological red-line, sensitivity of ecological environment, ecological service, prohibited development zone, Chishui River Basin