Abstract: Poyang Lake is the largest freshwater lake in China and an important international wetland. The ecological environment protection and sustainable development of this lake basin have great significance. In this study, GIS spatial analysis is used to figure out the non-point source adsorbed pollutants（nitrogen and phosphorus) of this region, which based on multi-source data in 1990, 2000 and 2008. Firstly, the soil erosion amount and modulus were estimated by the soil loss equation. Secondly, the lake sediments loadings were calculated by the sediments transferring ratios in the lake. Finally, the spatiotemporal variation of non-point source N and P loadings were simulated by Particulate N and P transfer empirical model. The results indicated that N and P pollutions were lower in the center but higher in surrounding areas. The pollutions were higher in the upper drainage areas but lower in lower areas, which was mostly due to the plains and hills are interactively distributed in this region and the low soil erosion intensities in this type of landforms. This region yearly N and P pollution order in the studied years is 2000 > 1990 > 2008.