Abstract: Land is the comprehensive carrier of production, living and ecological function (abbreviated as PLEF), and it should be the important foundation to conduct land quantitative evaluation and coupling and coordination characteristics analysis for the identification of land use conflict and the optimization of land space allocation. Based on the connotation analysis of PLEF, applying multi-scale fusion mathematical model, this research constructs the evaluation index system of PLEF, integrating both of the administrative unit scale and the grid scale, and the apply coupling coordination model to quantitatively calculate the coupling coordination degree in the study area of Jiangsu Province from 2005 to 2015. The result showed: (1) Land use production function (PF) and living functions (LF) in Jiangsu had been greatly improved from 2005 to 2015, for 13.79% and 21.72% respectively, but the spatial distribution differed obviously. PF and LF of the cities and counties in South Jiangsu were the highest, followed was Central Jiangsu, and Northern Jiangsu came the last; the ecological functions(EF) shows, a decline 6.03%. (2) Coupling coordination degree had been divided into 5 levels: well coordination area (WCA), intermediate coordination area (ICA), primary coordination area (PCA), substandard coordination area (SCA) and inferior coordination area (INCA). During 2005 and 2015, WCA and ICA increased by 13.79% and 21.72% respectively. PCA increased the largest by 22.05%, while SCA decreased the largest by 33.91%, separately. In addition, the proportion of INCA came the least by 2.5%.(3) The spatial and temporal distribution characteristics of coupling coordination of PLEF in Jiangsu were significant. South Jiangsu showed the highest coupling coordination level, Northern Jiangsu showed the largest change, and the whole province region put up a promotion of coupling coordination level from SCA to PCA. This research has realized the multi-scale PLEF evaluation at the regional scale, which might provide a reference for the identification of land use conflicts.