Abstract: The construction of ecological network pattern is one of the important ways to ensure regional ecological security, to coordinate economic and social development and ecological system contradictions, and to enhance human well-being. This study takes Anqing City as an empirical area, identifies the regional ecological sources by evaluating the ecosystem service functions, and then uses the circuit theory to construct the ecological network pattern, demonstrates the regional ecological network structure characteristics according to the current density, and finally proposes the regional ecological network and Ecological security optimization strategies. The results of this study indicate that the ecological network structure of Anqing City is mainly composed of 24 source sites larger than 1 km2, 3 potential corridors, 23 pinch points and 3 important obstacles. The ecological sources are mainly composed of forest land and cultivated land. The areas with better ecological resources are mainly distributed along the Dalongshan Forest Park in the north of the city, the edge area of the eastern development zone and the western farm area. The ecological resource flow in the central urban area is blocked, the ecological corridor connectivity is poor, and the ecological network pattern is significantly heterogeneous. This study analyzes the ecological network security under different thresholds, proposes the ecosystem improvement and restoration strategies under different network patterns, and has positive practical guiding implication for improving the supply capacity of regional ecosystem services and the connectivity of ecological networks.