Abstract: Based on the annual ichthyoplankton surveys under the Gezhouba Dam (GD) in the middle Yangtze River from 2007 to 2018, the assemblage of fish eggs and its temporal variation were studied using multivariate statistical analysis. This research aimed to investigate the change between the dominant species and the succession process in the species abundance and richness after the impoundment of the Three Gorges Reservoir (TGR). Over the past 12 years, 49 species belonging to 5 orders and 9 families were collected, and of which 28 species were drifting fish eggs. The species abundance varied from 18 to 30 between years and the egg richness increased significantly year by year. For the four major Chinese craps, the number and density of their eggs increased after an initial decrease. The diversity index of the fish egg community exhibit a slow downtrend, and three groups were identified using CLUSTER and nMDS. They were group A (2007-2008), group B (2009-2013) and group C (2014-2018). The SIMPER showed that the difference between groups was significantly and was mainly attributed to the turnover of the dominant species. Before 2013, The dominant species of fish eggs varied among Hemiculter Bleekeri, Squalidus argentatus, Xenocypris macrolepis and Ctenopharyngodon idellus. Since then, the eggs of Hemiculter. Bleekeri became the only dominant species. The results in our study showed that the species turnover became weak after the impoundment of the TGR, which indicated a structural decline in the fishery resources.