Abstract: Carrying out urban expansion simulation research plays an important role in optimizing the national spatial planning and practicing the concept of green development. Based on ecological sensitivity, with the support of RS and GIS technology, this paper selects Wuhan City for soil and water loss, habitat, geological disasters and water resources for sensitivity analysis, and conducts a comprehensive ecological sensitivity study in the study area, based on CA-Markov model and Wuhan phase theree land use data are used to simulate and predict the urban land use in 2025. Research results: Among the ecological comprehensive sensitivities in Wuhan, highly sensitive and extremely sensitive areas account for 32.52% of the study area. Based on the CA-Markov model, it is predicted that the urban land area of Wuhan will reach 1 845.00  km² in 2025; on the premise of respecting nature, by superimposing it with ecologically sensitive areas, the construction land area of Wuhan will be adjusted to 1 784.16  km². From the perspective of green development, this article guides the rational and orderly expansion of cities and guarantees the harmonious and stable development of social economy and ecological civilization.