Abstract:

Composition of bacterial community was investigated through directly extracted the environmental DNA and sequencing cloned 16S rRNA from the water in the Lao Ye Miao area of Lake Poyang.The relationship between the bacterial community and water physicalchemical characteristics of Lake Poyang was discussed.From October in 2006 and May in 2007 clone libraries，one hundred and eighty bacterial clones were classified into sixtysix operational taxonomic units (OTUs), most of which were affiliated to Alpha, Beta, Gamma, EpsilonProteobacteria, Bacteroidetes, Actinobacteria ,Planctomycetes, OP10, and Acidobacteria. BetaProteobacteria was the most significant lineage in 2006 October library, while GammaProteobacteria and Bacteroidetes were the most significant lineages in 2007 May library indicating that the bacterial community composition has seasonal characteristics. Phylogenetic analysis further showed that bacterial composition was highly similar with uncultured bacteria from freshwater and soil ecosystems, partial which related to degradation function