By improving the method of measuring accessibility and adopting adjusted gravity model, this paper collected the data of 11 cities of Jiangxi Province in 2010, 2013 and 2016, then measured the urban rail accessibility (URA) and interurban spatial effect (ISE) among cities, and analyzed the evolution of space-time pattern based on GIS. Research findings: (1) From the High-speed rail (HSR) "depression" to the HSR "highland", the layout of URA in Jiangxi Province takes Nanchang as the core, rail hub cities as the sub centers, and spreads to the surrounding areas with the "center-periphery" pattern. The ISE takes the main rail channels as the axes, and take the rail hub cities as nodes with the "point-axis" pattern. Both of them show the gradient differences between the north and south with the radiation diffusion to the suburb of Jiangxi Province. (2) HSR and regional integration are two important drivers to improve the URA. The huge compression of time and space weaken the influence of space distance on ISE, and promote the leap of ISE. URA and ISE show significant zonality and "channel effect", which are greatly affected by the density of operating trains among cities. (3) The impact range of HSR is limited, and its network effect in northern part of Jiangxi is stronger than that in central and southern part. The URA in the channel tends to be balanced, but it also widen the gap with the remote cities outside the channel, and the cities which haven’t been deeply integrated into the HSR network of province are at risk of being marginalized. (4) The ISE in Jiangxi Province shows obvious regional differentiation characteristics, forming three sub-systems in the middle, north and south. The difference of ISE is expanding, so the ISE in the province are in the pursuit of "efficiency". Based on this, Jiangxi Province should accelerate the construction and improvement of the rail and HSR network, design the scale of the stations rationally and distribute the train frequency accurately. Each city should actively be integrated into the HSR network of the province, and strengthen the local connection traffic construction and the integration with the surrounding cities, to enhance urban comprehensive transport accessibility and ISE. Jiangxi Province should co-ordinate north and south with gradient development, steadily promote regional integration, further break the geographical isolation and differentiation, to promote the balanced development between cities.