RESOURCES AND ENVIRONMENT IN THE YANGTZE BASIN >> 2016, Vol. 25 >> Issue (Z1): 38-43.doi: 10.11870/cjlyzyyhj2016Z1006

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STUDIES ON ARTIFICIAL PROPAGATION TECHNIQUES OF TRIPLOPHYSA XICHANGENSIS

LI Rong1, WU Xiang-xiang1, WU Shi-yong2, LI Yun-feng1, WANG Hong-mei2, RU Hui-jun1, SHENG Qiang1, ZHANG Yan1, GAN Wei-xiong2, CHEN Xiu-song2, NI Zhao-hui1   

  1. 1. Fishery Eco-environmental Monitoring Center in the Upper and Middle Reaches of the Yangtze River, Ministry of Agriculture/Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan 430223, China;
    2. Key Laboratory of Qionghai Wetland Environmental Protection in Xichang College, Xichang 615013, China
  • Received:2016-06-06 Revised:2016-06-14 Online:2016-11-26
  • Supported by:
    Project of Yalong River Hydropower Developmental Company(JPIA-G201224)

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Abstract: In this study we carried out a series of experiments on every key technologies of the artificial propagation of Triplophysa xichangensis, including oxytocin drugs and dosage, the insemination method, and the larval hatch and culture method. The results showed that, when the gonad of the female fish developed to the Ⅳ+-Ⅳ++ period, twice injection was given with 0.1 ml hormone containing LRH-A at a dose of 0.1 μg/g fish and HCG at a dose of 20 IU/g fish, while the doses were halved for male fish, it could preferably promote brood stock estrus and spawn. The response and effective time of the hormone was 30.5-31h at a water temperature of 19±0.5℃. All the artificial propagation processes of the fish, including the intensive cultivation of brood stock, hormone injection and natural spawning, hatching of fertilized eggs, cultivation of larvae fish, were carried out in a special plastic basin. The fertilization rate and hatching rate was 97.2% and 85%, respectively, and the survival rate of the 20d larvae fish was 85%. This research can be applied in large-scale breeding of Triplophysa xichangensis, and has positive significance for the protection of fish biodiversity in the upper Yangtze River.

Key words: triplophysa xichangensis, artificial propagation, oxytocin, fertilization, first feed

CLC Number: 

  • S96
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