Abstract: To investigate and understand soil selenium concentrations with its bioavailability and influencing factors in Shadi Town, Enshi City, Hubei, which is a known seleniumenriched area, 199 topsoil samples and 60 corn samples were collected and analyzed. Results showed that the average concentration of total selenium in the topsoil of Shadi Town, Enshi City was 1.88 mg/kg, whereas the concentrations of watersoluble selenium in soil reached 004%-291% of the total soil selenium indicating the relatively low level of selenium. In the study area, the concentrations of soil organic carbon showed minor effects on selenium bioavailability. The main influencing factors of selenium bioavailability included the soil total selenium concentrations and soil pH value owing to the high selenium bioavailability in alkaline soil. As considerable portions of topsoil samples were acidic, and the concentrations of soil watersoluble selenium were low in the study area, the low selenium concentrations were observed in the corns. Soil pH value can be adjusted to improve selenium bioavailability