Abstract: Urban sprawl squeezes the natural environment space on scale, and structurally increases the degree of habitat fragmentation. In addition, it has a negative impact on the cities’ ecological neighborhood, which should be taken into consideration when measuring the change of ecological service value caused by urban sprawl. This paper took Nanchang city as the study area and measured the change of ecological service value. On this basis, we probed into the interference effect of different types of construction land, and estimated the ecological service value enhanced by introducing the differentiate interference effect. Thus we compared the change of ecological service value from 1995 to 2005 with that from 2005 to 2015, and then made an in-depth analysis of differences before and after method improvement. The results showed that, the interference intensity of urban sprawl patches in Nanchang was relatively high in general. The interference effect of urban sprawl between 2005 and 2015 was obviously higher than that between 1995 and 2005, which indicated that the ecological cost of urban sprawl was increasing. Second, the spatial distribution of interference effect had significant difference. To be specific, the urban expansion patches surrounded by more cropland, woodland and waters presented higher interference effect. Third, the change of ecological service value estimated by improved method was larger than that without improvement. For the urban sprawl patches with different composition of neighborhood or different types of source, significant difference was observed in the change of ecological service value. We believe that the method and its application in the study area can aid in measuring the change of ecological service value caused by urban sprawl, and thereby provide technological support for estimation of ecological cost in the course of urban expansion.