Abstract: Pollination service is one of the most important ecosystem service for preserving wild plant communities and support agricultural production. Growing demand for food and corresponding intensification of agricultural production has widely been recognized a major threat to pollination services provided by insects. Since two-thirds of the world’s major food crops are pollinator-dependent. The decreasing of pollination service will lead big threads of food security. However, the correlations between ecosystem pollination services and land intensification should be verified in all over the world, especially in big agriculture country such as China. In this study, we investigate correlations between ecosystem pollination services and land intensification in village scale. We selected a village in the Poyang Lake region in Eastern China to analysis land use change impacts on pollination services for the period of three years (2000-2013). We used high-accuracy land use data, pollinating bees’ biophysical data, districts’ social economy and the data from field investigation. For the impact analysis, we employed the “Integrated Valuation of Ecosystem Services and Trade-offs - InVEST” model, a GIS-based tool, in combination with regression analysis to quantify the relationship between pollination service and land use intensities. Result showed that (i) the case study village received an average pollination service abundance rate of 0.127 and 0.108 respectively while the average pollination service abundance decreasedby15.5% during 2000-2013, (ii) farm land at the village level was reduced by 13.5% whereas farm investments for pesticides, chemical fertilizers and labor forces doubled during the same time of period, and (iii) regression coefficient analysis shows a negative correlation between land intensification and pollination service abundance of -0.164. That also means the there is as trade-off correlations between pollination services and intensification of agricultural production. Based on our results, we conclude that the land conservation does not offset the big negative effect on pollination services. Instead the substantially increase of land intensification due to pesticide applications has negatively affected the bee pollination effects in the case study village.