Abstract: Based on the land utilization data of the Yangtze River Delta urban agglomerations in 1990, 2000, 2010 and 2015, the land utilization distribution pattern and the transfer direction of the Yangtze River Delta urban agglomeration have been described using the transition probability matrix of land utilization methods, Then, the value of ecosystem services within the region quantitatively was analyzed by the Xie Gao-di modified Costanza value evaluation methods, and its spatial distribution pattern was explained by geodetector. The research showed that the main land utilization type of urban agglomeration in the Yangtze River Delta was cultivated land, showing a trend of decreasing year by year. The change of woodland was relatively stable, the change of water area was increased in the early time and then decreased, the construction land has been growing, and the retention rate of construction land was the top one, which cultivated land was the main source of conversion, grassland was mainly converted into cultivated land and forest land, and the water were mainly transferred to construction land. The ecosystem services value in the 1990, 2000, 2010 and 2015 of Yangtze River Delta urban agglomerations was 266.426 billion yuan, 263.953 billion yuan, 261.549 billion yuan and 256.988 billion yuan, showing a trend of decreasing year by year. The value of farmland ecosystem decline was most obvious, and the contribution of forest ecosystem makes the greatest contribution to the entire region The value of the ecosystem services of cities with grades III and IV is higher than that of I and II, and the larger the scale of the city, the more obvious dominant position of the economic value. The spatial distribution pattern was characterized by high-value clusters centered on Taihu Lake and Chaohu Lake, and higher in the southeast region than in the northwest region, which influenced by slope, elevation, soil type and population.