Abstract: Abstract：Response of spatio-temporal evolution characteristics of habitat quality to land cover change and landscape pattern change in Anhui Province was studied based on ArcGIS platform and the InVEST model, using the land use data of Anhui Province in 1995, 2005, and 2018.The main conclusions are the following: The main land cover changes in Anhui were that cultivated land was converted to construction land, forest and grassland, and there was mutual conversion between wetland and cultivated land.During the study period, The average index of habitat quality in Anhui Province showed a slight one-sided decline trend.Due to the rapid expansion of construction land which acted as a threat factor, habitat quality declined dramatically in urban and surrounding areas of Hefei and 5 cities along the Yangtze River.The effective project of returning farmland to lake and grain for green were carried out in some protected areas and surrounding areas, there has been a marked improvement in the habitat quality while the change of habitat quality in most ecological source areas was not obvious on a regional scale.The paper also puts forward policy suggestions for the optimization of habitat quality in relative areas.