Abstract: New infrastructure construction is an important choice for urban construction in the critical stage of the transformation from extensive epitaxial development to intensive green convolution. Based on the the panel data of 33 cities in the upper reaches of the Yangtze River from 2008 to 2019, this paper from the perspective of the “Production-Living-Ecological” Space, explores and discusses the mechanism of the new infrastructure construction and the urban green transformation by using the OLS regression, spatial measurement and stepwise regression methods. The results showed that: （1) First, the new infrastructure construction could enable the urban green transformation in the upper reaches of the Yangtze River, and the enabling effect on the transformation of ecological space was more prominent than the transformation of production space and living space; (2) Second, the new infrastructure construction had a spatial spillover effect on urban green transformation, and the spatial factors had accelerated effect, which strengthens the positive effect of new infrastructure construction on urban green transformation；（3) Third, through green technology innovation effect, enterprise digitalization effect and environmental regulation orientation effect, new infrastructure construction had a differentiated effect on the “Production-Living-Ecological” space transformation, and then promoted urban green transformation of cities. Among them, green technology mainly drove the transformation of production space, enterprise digitalization focused on enabling the transformation of living space, and environmental regulation promoted the transformation of ecological space. Based on the above conclusions, we proposed the following suggestions: building a new ecological infrastructure system, strengthening green technology innovation, accelerating enterprise digitization, and implementing diversified environmental regulation tools.