Abstract: The Three Gorges Reservoir Area(TGRA) is an important ecological barrier area and an ecologically fragile area on the Yangtze River Economic Belt. It is of great research value and significance to construct an ecological safety network by extracting important ecological sources, constructing comprehensive ecological resistance surface, identifying important ecological corridors and ecological nodes in the TGRA. Taking the TGRA as the study area, morphological spatial pattern analysis (MSPA) was used to identify forest ecological source patches, and the importance of patches was evaluated by landscape connectivity index. Combined with the resistance factors of land use type, DEM, slope, distance from river, distance from road and NDVI, the comprehensive ecological resistance surface was constructed.The minimum cumulative resistance model(MCR) was used to construct the ecological corridor, and the important ecological nodes were extracted to form the forest ecological safety network in the TGRA. The results of the study showed: (1) There are 38 ecological sources of forest land in the TGRA, accounting for 59.55% of the forest area and 27.95% of the total area of the region. They are mainly distributed among the Daba-Wushan-Wuling Mountains in Hubei and Jinyun-Zhongliang-Tongluo -Mingyue -Yunwu Mountains in Chongqing.(2) High resistance areas are mainly distributed in urban construction areas, the highest value of resistance is located in the main urban area of Chongqing; low resistance areas are mainly distributed in woodland and water areas, and the lowest value of resistance is located in the Wushan mountainous area in the Hubei section of the TGRA. (3) There are 79 ecological corridors, including 3 important corridors and 76 general corridors. The total length of the corridors is 1 326.37 km. (4) The average resistance of ecological corridors is 2.50, and the total cumulative resistance of corridors between ecological patches is 197.36; α index, β index, and γ index are 0.52, 2.08, and 0.73 respectively; cultivated land, woodland, and grassland are the main landscape type of corridors. The results of the research present an ecological network spatial pattern of “three screens, multiple corridors, multiple spots, and multiple patches”, providing scientific guidance for protecting biodiversity, improving ecosystem functions, and maintaining regional ecological security patterns.