Abstract: Industrial revitalization is the foundation and key to achieve a comprehensive rural revitalization. The evaluation index system of rural industry revitalization was constructed from three dimensions: revitalization of agricultural product industry system, revitalization of multi-functional agricultural industry system and revitalization of agricultural support industry system. The entropy weight TOPSIS method was used to measure the level of rural industry revitalization in 25 prefecture-level cities in the Huaihe River Ecological Economic Belt (HREEB) from 2013-2022. The obstacle degree model was used to diagnose the obstacle factors of rural industry revitalization of the region. The study showed that, the level of rural industrial revitalization in the HREEB was relatively low. The comprehensive score fluctuated in the range of 0.2~0.4, showing an upward trend. There were strong spatial and dimensional differences in the revitalization level of rural industries among the three major regions of the HREEB , with a concave distribution characteristic of "high at both ends and low in the middle". Among the three dimensions, the revitalization of agricultural products industry system and the revitalization of multi-functional agricultural industry system had relatively high comprehensive scores, but the revitalization of agricultural support industry system had lower comprehensive scores. Further diagnosis of the obstacle model concluded that the imperfect construction of rural digital infrastructure, weak rural energy input and support capacity, and insufficient cultivation of new types of business were the main factors restricting the revitalization of rural industries in the HREEB. Countermeasures were proposed in terms of strengthening cross-regional synergy among cities along the Huaihe River, promoting rural digital infrastructure construction, strengthening rural energy revolution, and fostering the development of new industries and new business forms in the countryside.