RESOURCES AND ENVIRONMENT IN THE YANGTZE BASIN >> 2007, Vol. 16 >> Issue (3): 314-314.

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RAPD ANALYSIS OF GENETIC DIVERSITY OF CYPRINUS CARPIO AND CARASSIUS AURATUS IN NANJISHAN NATURE RESERVE IN POYANGHU LAKE

HU Maolin1, WU Zhiqiang1, CHANG Jianbo2   

  • Received:2006-03-07 Revised:2006-06-16 Online:2007-05-20

Abstract:

The colonial genetic variation in Cyprinus carpio and Carassius auratus was analyzed with RAPD technique. Eight primers were screened out from 40 random primers on the genomic DNA in Cyprinus carpio. A total of 60 bands were generated, of which polymorphic bands were 42, with the percentage of polymorphism being 70.00%. Using the 8 primers for genomic DNA of Carassius auratus, 61 bands were generated, of which 40 were polymorphic, with the percentage of polymorphism being 65.57%. As analyzed by software POPGENE, the colonial genetic diversity was both high in Cyprinus carpio (He=0.230 1, H0=0.391 0) and Carassius auratus (He=0.218 6, H0=0.375 8). However, in the population of Cyprinus carpio and Carassius auratus, the colonial genetic diversity was very similar in females and males.

Key words: Poyanghu Lake, Nanjishan nature reserve, Cyprinus carpio, Carassius auratus, RAPD

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