Abstract: Under the background that Central Government vigorously promotes the new type urbanization and optimizes the strategic pattern of territorial development, urban agglomeration area has become the core carrier of interregional competition. Quantitative cognition of the long-term spatio-temporal pattern of urban expansion of urban agglomeration area and the pattern variation law of cities at different scales is of important significance for improving the urban governance capacity and formulating appropriate spatial control strategy. Based on the land use data in four stages(1990-2000、2000-2005、2005-2010 and 2010-2015) of Wuhan Urban agglomeration during 1990-2015, this research on spatio-temporal pattern of urban expansion of Wuhan Urban agglomeration and pattern variation law of cities at different scales could be conducted by indexes including the urban land sources, urban expansion rate, urban expansion index, center of gravity coordinates, fractal dimension and expansion intensity equal fan analysis. Results show: the main source of urban land expansion in Wuhan urban agglomeration is farmland, followed by water, woodland, other construction land and rural settlements, which tends to change from a single source to diverse sources. Urban land expansion rate shows obvious characteristics of fluctuation and stage; the expansion appears spatial heterogeneity and spatial agglomeration. Regions with medium and fast expansion speed in different periods exhibit different shapes, such as a fan or “U” type. According to the radar chart, expansion pattern of urban land use could be summarized into three modes, one-wing mode, multi-wing mode and fan-shaped mode. The main expansion directions of each cities show no uniformity and emerge a feature that small cities gather towards big cities. The shape of Wuhan urban agglomeration is regular, the fractal dimension appears a characteristic of fluctuation. The barycentric coordinates of Wuhan, Huangshi and Xianning develop towards the southwest; that of Ezhou, Huanggang and Xiaogan develop towards the northwest. There are differences among the urban expansion patterns and modes at different scales. The expansion speeds of the small and medium-sized cities are larger than that of the big cities, and the urban morphology tends to be stable.