Abstract: Based on the land use data of the 6th period of Zhejiang Province from 1990 to 2015, the temporal and spatial changes of land use are analyzed, and the ecological risk index is constructed to evaluate the ecological risk of land use in Zhejiang Province in different periods. The results show that: (1) The land use change in Zhejiang Province is characterized by a large reduction in arable land and a sharp increase in construction land; the direction of land use transfer is mainly the transfer of cultivated land, forest land, and water areas to construction land; of which, the land use dynamic degree is the highest from 2000 to 20005 and from 2010 to 2015; (2) The quality of ecological environment in Zhejiang Province has declined. the moderate ecological risk area is the dominant type of area, and the high and the extremely high ecological risk areas expand outward. The high value areas are mainly distributed in the vicinity of Hangzhou Bay, the coastal area, and the areas bordering the southwest of Hangzhou, Quzhou and Jinhua. The low-grade ecological risk areas are distributed around the adjacent high-level ecological risk areas and generally spread out in a circular pattern. (3) Land use ecological risk grade conversion varies greatly. The ecological risk shift is mainly from low to high level, and the annual average conversion rate of its ecological risk level is on the rise. Ecological risk conversion mainly occurs in cities and coastal areas such as Hangzhou Bay, Wenzhou, and Taizhou coastal areas where economic development is active. (4) The ecological risk values in the cities of different levels in Zhejiang Province differ greatly in time and space. The city’s ecological risk values mainly show an upward trend, Ningbo’s ecological risk changes the most, and Quzhou City has the smallest change. In terms of spatial distribution, Hangzhou has the highest ecological risk value, which is much higher than other prefecture-level cities. Zhoushan has the lowest ecological risk value.