Abstract: In order to optimize urban ecological network structure of Shanghai city, some ways to improve urban ecological quality are proposed incorporating landscape pattern, landscape pattern index and landscape ecological network analysis. First, landscape types such as construction land, agricultural land, water area and green land during 2008 and 2015 was obtained by using object-oriented remote sensing image classification method, and the landscape pattern index are calculated using Fragstats software. The evolution of urban landscape pattern and landscape pattern index in the above two years were compared and analyzed. Then, the least cost path method is used to develop the urban ecological network and overlap analysis between the ecological network and the urban road network is used to determine the break points of the ecological network structure. Finally, compared with the plan of Shanghai Basic Ecological Network Planning, the suggestions for the future development of the urban ecological network are put forward. The conclusions are as follows: (1) The proportion of Shanghai construction land, agricultural land, green land and water area changed from 38:51:5:6 to 46:39:7:8. (2)  The landscape pattern of Shanghai is becoming fragmented and complicated, and the connectivity is declining; the dominant landscape type of construction land is strengthened; the suburban construction land is fragmented; the agricultural land is reduced and fragmented; the green land is enlarged, but the connectivity is insufficient, and the structure needs to be optimized. (3) 105 potential eco-corridor lines in Shanghai were obtained by the least resistance analysis, which had better circularity in Pudong New District, Fengxian District, Jinshan District, Songjiang District and Qingpu District, and 62 break points of the main eco-corridor in Shanghai were identified by overlay analysis, mainly distributed in Qingpu District, Songjiang District, Pudong New District and Fengxian District. (4) Through comparative analysis, it is found that the framework of the ecological network of Shanghai Basic Ecological Network Planning has basically been maintained, but the local ecological network structure is facing problems. The space of the ecological corridors in the northwest and north of Shanghai is shrinking, and the ecological space in the southeast is fragmented.