Abstract:

This experiment used NH4Cl for the main constituent to prepare ammonia solutions and a Y shape water maze for experiment equipment.Our purpose was to find out the selection of P〖WTBX〗rocambarus clarkii〖WTBZ〗 of different ammonia concentration from the ethological point of view.Five concentrations of 4750,7125,9500,14250 and 19000 mg/L were set.Their pH value was 70,the temperature was 20±05℃.〖WTBX〗P.clarkii〖WTBZ〗 subadult of 501±043 cm in body length and juvenile of 075±015 cm in body length were used for the experiment.Ammonia solution and clean water were put in the 2 selection areas of a Y shape water maze,then let the crayfish choose,the times of selection of ammonia solution,clean water and no selection were recorded.Binomial test of nonparametric tests to calculate the data was carried out.The result indicated that the subadults had no selection at the ammonia concentration of 4750,7125,9500 and 14250 mg/L,but they significantly avoided the ammonia solution with concentration of 19000 mg/L;the juveniles had no selection at the ammonia concentration of 4750 mg/L,but they significantly avoided the ammonia solution when the ammonia concentration is 7125 and 9500 mg/L,and they had toxic symptom and lost the ability of selection when the ammonia concentration is 14250 and 19000 mg/L.The study indicated that 〖WTBX〗P.clarkii〖WTBZ〗 has no preference for ammonia solution and will avoid the ammonia solution when the concentration reaches the right value or even die when they can't avoid in time