Abstract: In order to explore the air flow variation characteristics of karst cave, to reveal the temporal and spatial variation and the change mode of the ventilation effect in karst cave. With two monitoring cycle during January 2016 to June 2019 , this study had a 32-month data collection about the temperature, relative humidity, CO2 concentration and pressure in Mahuang Cave which located in Suiyang. Base on the mode to analysis, the result shows that there are three ventilation modes in the Mahuang Cave, the limited ventilation mode in the summer, the active ventilation mode in the winter and the transition phase in the spring and autumn. The circulate of air flow is restricted in the summer, active in the winter and some exchange in the spring and autumn. The structure of the cave affects the ventilation mode and the ventilation effect also affects the microclimate. According to the influence of the ventilation effect, the Mahuang Cave can be divided into three parts: 1#-5# monitoring point with air exchange, 6#-9# monitoring point with turbulence and 10#-15# monitoring point with relative stable air environment. Due to the special structure of the cave, there is "gorge wind " caused by " Canyon effect " in the middle of the cave, and there is obvious airflow in the middle of the cave in spring and autumn. Analysis of karst cave ventilation mode and study the mode and variation character of ventilation effect in karst cave, it is of great significance to the study of sediment growth, climate change and carbon cycle model.