Abstract: As an important carrier of rural revitalization and urban and rural leisure tourism, systematic analysis of the spatial distribution and accessibility of national-level villages is helpful to make rational use of rural resources and promote the comprehensive development of regional rural tourism. This study took five types of national-level villages in the Yangtze River Economic Belt, namely historical and cultural villages, traditional Chinese villages, ethnic minority characteristic villages, forest villages and key villages of rural tourism, as the research objects. The GIS spatial analysis method and mathematical statistics method were used to describe the spatial distribution and accessibility difference pattern of the villages from different spatial scales. The results showed that: (1) The spatial distribution types of the five types of villages all had typical agglomeration characteristic. The agglomeration intensity showed a sequence pattern of "traditional villages> ethnic villages > forest villages> historical and cultural villages> key rural tourism villages "; (2) The non-equilibrium degree of cities at the prefecture level and above in the five types of villages was higher than that at the provincial level, and the balance degree of quantity distribution was poor; (3) The spatial accessibility of the five types of villages was significantly differentiated. The internal differences were prominent, except for the accessibility of ethnic minority characteristic villages, which was "strong in the west and weak in the east". The accessibility of the other four characteristic villages showed a spatial pattern of "high in the east and low in the west"; (4) The accessibility of county units in five types of villages was affected; (5) Under the case of one-day, two-day, three-day, five-day and seven-day tours, the cities with daily accessibility advantages were identified as Hangzhou, Nanchang, Nanchang, Guizhou and Guizhou