长江流域资源与环境 >> 2021, Vol. 30 >> Issue (11): 2703-2711.doi: 10.11870/cjlyzyyhj202111013

• 生态环境 • 上一篇    下一篇

低剂量Cd对崇明东滩湿地不同覆被类型土壤反硝化速率的影响

徐传红1,韩金1*,程虎1,韩建刚1,2,3*   

  1. (1.南京林业大学生物与环境学院,江苏 南京 210037;2.南京林业大学南方现代林业协同创新中心,
    江苏 南京 210037;3.江苏洪泽湖湿地生态系统国家定位观测研究站,江苏 洪泽 223100)
  • 出版日期:2021-11-20 发布日期:2021-12-14

Effect of Low Dose Cadmium on Denitrification Intensity of Soil  with Different Covers in Chongming Dongtan Wetland

XU Chuan-hong1, HAN Jin1, CHENG Hu1, HAN Jian-gang1,2,3   

  1. (1. College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China; 2. Co-Innovation 
    Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; 
    3. National Positioning Observation Station of Hung-tse Lake Wetland Ecosystem in Jiangsu Province, Hongze 223100, China)
  • Online:2021-11-20 Published:2021-12-14

摘要: 全球变暖及人为活动加速影响下,重金属对湿地生态系统反硝化(Den)的影响研究备受关注,但目前有关不同覆被类型下低剂量重金属的效应评估还很不充分。本文以崇明东滩湿地为例,采集光滩、互花米草(Spartina alterniflora)和芦苇(Phragmites australis)3种覆被下的土壤样品,外源添加不同剂量(0、0.05、0.1、0.3、0.5、1.0、3.0和5.0 mg·kg-1)的Cd,借助15N同位素示踪技术观测土壤Den速率的变化。结果表明:芦苇覆被下土壤Den速率(9.2±0.3 μg·kg-1·h-1)>互花米草(4.7±0.5 μg·kg-1·h-1)。Cd的添加导致两种植被下土壤Den速率均显著降低(P<0.05),芦苇和互花米草下土壤Den速率分别降至6.3和3.2 μg·kg-1·h-1。Cd胁迫下,Den速率的变化表现为剂量驱动下的“阶梯状”特征。即,低剂量(0.05~0.3 mg·kg-1)区间,Den的抑制率稳定保持在13.3%~14.8%。当Cd的剂量上升至0.3~0.5 mg·kg-1时,Den的抑制率从14.8%增至50.0%。高剂量(0.5~5.0 mg·kg-1)下,Den的抑制率再次趋于稳定(50.0%~55.0%)。相关分析表明,Cd胁迫下,芦苇和互花米草植被下土壤NO3--N含量与Den速率显著正相关(P<0.05)。这表明,低剂量Cd的存在并未改变NO3--N对Den过程的主导。


Abstract: Effects of heavy metals on soil denitrification rates in wetland ecosystems has attracted much attentions with accelerated global warming and anthropogenic activities, but few studies have evaluated the low-dose effects under various plant types. In the present study, soils were collected from bare beach, Spartina alterniflora and Phragmites australis in Chongming Dongtan wetland, Shanghai, China. Gradient doses of Cd (0, 0.05, 0.1, 0.3, 0.5, 1.0, 3.0 and 5.0 mg·kg-1) were added into the soils, and the Den rates were determined based on 15N isotope tracer technology. The results showed that mean Den rate in soils with Phragmites australis (9.2±0.3 μg·kg-1·h-1) was higher than that of Spartina alterniflora (4.7±0.5 μg·kg-1·h-1). The presence of Cd resulted in the significant decrease of Den rates under the two vegetation (P<0.05). The soil Den rates under Phragmites australis and Spartina alterniflora decreased to 6.3 μg·kg-1·h-1 and 3.2 μg·kg-1·h-1, respectively. Under Cd stress, the change of Den rate showed a "step-like" mode with doses. In the low dose (0.05-0.3 mg·kg-1) interval, the inhibition rate of Den rates remained stable at 13.3%-14.8%. When the dose of Cd increased to 0.3-0.5 mg·kg-1, the inhibition rate increased from 14.8% to 50.0%. At high doses (0.5-5.0 mg·kg-1), the inhibition rate reached a higher level and stabilized again (50.0%-55.0%). Correlation analysis showed that the soil NO3--N contents were significantly positively correlated with Den rates under Spartina alterniflora and Phragmites australis (P<0.05). This indicated that the presence of low-dose Cd did not change the dominance of NO3--N on the Den process in the coastal wetland. 

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