Abstract: It is of great significance to explore the coupling coordination relationship and evolution trend of the agricultural eco-economic system for scientific evaluation of ecological governance benefits and realization coordinated development of agricultural eco-economy in the karst area. Taking Guizhou Province as an example, based on the data of agricultural ecological environment and agricultural economic development, this paper constructs the evaluation index system of agro-ecological system and agro-economic system. Using entropy weight method, coupling coordination degree model and coupling degree model, we quantitatively analyzed the comprehensive evaluation index, state of coupling coordination, and trend of coupling degree evolution of the agro-ecological system and agro-economic system in Guizhou Province from 2000 to 2019. The results showed that the comprehensive evaluation index of agro-ecological system presents a trend of first decreasing and then increasing in Guizhou Province, but the agro-economic system maintains a steady rising trend, and the relationship between the two is gradually evolving from “economic lag type” to “ecological lag type”. The coupling coordination index of agricultural eco-economic system increased from 0.268 0 to 0.660 6, besides the coupling coordination state developed from the moderate imbalance level to the primary coordination level. The coupling degree presents a trend from large to small, and the system has experienced a change from depletion development to coordinated development, which indicates the agricultural eco-economic system of Guizhou Province showing a trend of coordinated development. Our results indicate that Guizhou Province should continue to practice the concept of ecological priority and green development, optimize the relationship between ecological environment and economic development and realize the high-level coordinated development of agricultural eco-economic system.