Abstract: The Tuojiang River watershed is located in the middle of Sichuan Basin where the human and water environment frequently interact with each other. In recent years, the water environment pollution and degradation of ecosystem services are becoming more and more seriousowing to rapid population growth, fast economic development and accelerated urbanization process lead to in this watershed. This study adopted the assignment of ecosystem service value per unit land use area and export coefficient as approaches, then estimated the ecosystem service values and pollution loads of 28 counties in Tuojiang River watershed, and finally revealed their matching relationships from spatial scale toto provide suggestions for water environmental management and ecological conservation. The results included following aspects:（1）Comparing individual pollutant emitted amounts, the COD emission was largest, TP emission was lowest, and the maximum values of COD, NH3-N, TN and TP pollution loads were 71 067.51 t, 111 08.76t, 21 320.08 t and 3 073.99 t, respectively. The distribution of pollution load had great spatial heterogeneity in this study watershed. The pollution load in the upstream was smaller, the pollution load in the middle reaches was larger, and the pollution load in the downstream was lower than that in the middle reaches.（2）The spatial differentiation of the four types of ecosystem service values in the basin was obvious. The larger ecosystem service values of provisioning, regulating, supporting and cultural ecosystem services values occurred in upstream of study watershed. The spatial distribution pattern of total ecosystem service value was very similar to that of supporting ecosystem service.（3）The spatial differentiation of the matching relationship between ecosystem service value and pollution load in Tuojiang River watershedwas also obvious.The higher values of spatial differentiation appeared in the middle and downstream of watershed. Therefore, it is necessary to build a reasonable ecological compensation mechanism and effective pollution load reduction strategies to improve the water environment and ecosystem service functions in these districts.The study on spatialrelationships between pollution loads and ecosystem services values not only deepens the understanding of the relationship between ecosystem services and pollution loads, but also enriches the research case of water environmental management in the watershed. It provide the basis of water environmental management and ecological construction.