Abstract: The official issuance of the Outline of the Development Planning of the Yangtze River Economic Zone has pointed out the direction of regional economic and industrial development, and its industrial transfer and regional cooperation have gradually become the focus of geographical research. Based on the total industrial output value and the number of enterprises of 28 manufacturing industries in 11 provinces and cities of the Yangtze River Economic Zone in 2008 and 2017, this paper explores the transfer of manufacturing industries and regional cooperation in the Yangtze River Economic Zone by means of dynamic agglomeration index, shift-share analysis method (SSM) and static agglomeration index. The research shows that: (1) The labor-intensive and capital-intensive manufacturing industries such as the textile industry, non-ferrous and ferrous metal smelting and rolling processing industry in the Yangtze River Economic Belt have strong spatial transfer characteristics. Their transfer paths are many-to-many diffusive and jumping transfers from Shanghai, Zhejiang to Guizhou and Hunan in the middle and upper reaches of the Yangtze River; The main types of transfer are “advantage transfer type”, “elimination type” in downstream areas and “advantage agglomeration type” in middle and upstream areas.(2) The development of transportation, chemical manufacturing and other industries in the upper and middle reaches of the Yangtze River Economic Zone is relatively weak, and lack of central cooperation areas, which requires more technology financial support from the downstream areas; (3) As the supplier of industrial transfer and technology transfer, the lower reaches of the Yangtze River Economic Belt should be built as the manufacturing transfer center, the middle and upper reaches with industrial comparative advantages as the regional center, increasing the transfer and cooperation of manufacturing industry to the middle and upper reaches of the Yangtze. The research will help to improve the theory of manufacturing transfer and regional cooperation, and provide reference for the manufacturing cooperation and coordinated development of the manufacturing industries in the Yangtze River Economic Belt.