Abstract:  The increasing shortage of cultivated land resources drives profound changes in cultivated land multifunction. 63 counties in Jiangsu Province were selected as study units. This paper constructed the multi-functional evaluation index system of cultivated land from four dimensions of production, ecology, landscape and society. Sensitivity analysis model and cultivated land index were used to analyze the spatial and temporal evolution of cultivated land functions and its sensitivity to cultivated land change from 2000 to 2018. The results showed: (1) Production function of cultivated land in Jiangsu Province increased. Spatially higher in the north and lower in the south, and the high-value areas in Northern Jiangsu continued to spread. Ecological functions declined. The function of Central Jiangsu was high. Due to the improvement of agricultural technology, some areas of Central Jiangsu and Northern Jiangsu had gradually changed from decline to increase. Landscape function increased, spatially higher in the east and lower in the west. The function of Eastern Coastal area was high. In some areas, such as Suzhou, Wuxi and Changzhou, due to the serious decline of cultivated land, the function has changed from rising to falling. The social function declined, spatially higher in the north and lower in the south. But the high-value areas in northern Jiangsu continued to shrink.(2) The overall cultivated land area of Jiangsu Province declined, gradually intensified from north to south. The relatively sparse area of cultivated land was mainly distributed in Southern Jiangsu while the agglomerate area was mainly distributed in Central and Northern Jiangsu. Cultivated land of Eastern Coastal area was the most concentrated.(3) Compared with 2000- 2010, sensitivity of production function decreased during 2010-2018, whereas the sensitive area shrank to three counties in Eastern Coastal area. Sensitivity of ecological function decreased, whereas the sensitive area shrank to southern Jiangsu. The decline of production function and ecological function were related to agricultural technology and intensive use of cultivated land. The sensitivity of landscape function increased, whereas sensitive areas spread to Southern Jiangsu and Eastern Coastal areas. The sensitivity of social function has increased, whereas sensitive areas spread throughout the province. Both of landscape function and social function were sensitive to changes in the amount of cultivated land.