Abstract: The Chongqing section of Three Gorges reservoir area was used as the case study area. From the perspective of ecological security，ecological sources were identified via evaluation of ecological sensitivity and ecosystem service value. Ecological security pattern was built via the Minimum Cumulative Resistance model. Based on Ecological security pattern，the land use conflict was identified of the Chongqing section of Three Gorges reservoir area in 2015. we expect the research results will provide reference for the land use planning and management. The results show that: (1)The spatial distribution of cultivated land conflict was relatively scattered，the type of very serious conflict was mainly distributed in Fengjie，Kai，Shizhu and Fuling counties. The type of serious conflict and lower serious conflict were mainly distributed in the peripheral counties of the main city of Chongqing. The type of general conflict and non-conflict were mainly distributed in Changshou and Banan counties;(2)The spatial distribution of construction land conflict was relatively concentrated，the type of very serious conflict was mainly distributed in the main city of Chongqing. The spatial distribution of serious conflict and lower serious conflict were relatively scattered. The type of general conflict and non-conflict were also mainly distributed in the main city of Chongqing，and located on the periphery of very serious conflict;(3)The type of very serious integrated land conflict was mainly distributed in the central of the main city of Chongqing and Fengjie county. The type of serious conflict and lower serious conflict were distributed in every county. The type of general conflict and non-conflict were mainly distributed in the peripheral areas with very serious conflict.