Abstract: Healthy ecosystem and harmonious development of man-land relationship are of great significance to guarantee the water quality in Danjiangkou reservoir area and the smooth operation of the Middle Route Project for South-to -North Water Transfer in China.Taking Danjiangkou reservoir area, the water source of the Middle Route Project for South-to-North Water Transfer as the research area. Getting multi-temporal remote-sensing images (1991, 2000,2010, 2018）in the Danjiangkou reservoir area, the researchers interpreted the images to obtain the land use data using the object-oriented classification method based on samples. Building the model of ecosystem services value assessment, ecosystem service flow gain-and-loss matrix and the human activity intensity index assessment based on the land use data. In order to explore the characteristic of spatial-temporal correlation between the two in Danjiangkou reservoir area, the human activity intensity index was correlated with ecosystem services value by bivariate spatial autocorrelation method. The results indicated that: In Danjiangkou reservoir area, the construction land, woodland , reservoir and rivers showed an overall growth trend, while the farmland, shrub grassland, ponds, wetland and unused land showed an overall decrease trend from 1991 to 2018. The ecosystem services value in Danjiangkou reservoir area increased by 43.93×108 yuan and it showed a fluctuating trend between 1991 and 2018. The loss of the ecosystem services value in Danjiangkou reservoir area mainly came from the conversion of woodland into farmland and construction land, and shrub grassland into farmland. The gain of the ecosystem services value in Danjiangkou reservoir area was mainly from the conversion of farmland into woodland, reservoir and rivers. In terms of the intensity distribution of human activity in the danjiangkou reservoir area from 1991 to 2018, low intensity areas were the main. The medium-high intensity areas were clustered in the east of the reservoir, the high-intensity areas were clustered in small pieces and have been increasing. And there was a negative relationship between the ecosystem services value and the human activity intensity in Danjiangkou reservoir area from 1991 to 2018, and it showed a trend of first strengthening and then weakening.