Abstract: The study on the intensive use of industrial land in development zones has an important impact on improving land use efficiency and optimizing industrial layout. This paper takes industrial enterprise land in Jiangsu Province as the research object, adopts the updated survey data of industrial enterprises in Jiangsu Province in 2020, compares the spatial differences and industry differences in the intensive use of industrial enterprise land inside and outside the development zones in Jiangsu Province, and uses the multiple linear regression model to analyze the influence of whether enterprises enter the park on the intensive use of industrial enterprise land. The results show that: (1) The floor area ratio, building density, taxes per hectare and employees per hectare of industrial enterprises in the development zone are higher than those outside the development zone, the level of intensive land use by industrial enterprises inside and outside the development zone is higher in southern Jiangsu than in central and northern Jiangsu. (2) There are industry differences in the intensive use of land by industrial enterprises inside and outside the development zone. The land use intensity of equipment manufacturing and raw material processing industries in the development zone is significantly higher than outside the development zone, and the land use and social benefits of most industries in the development zone are significantly higher than outside the development zone. (3) Factors such as enterprise admission, enterprise investment intensity, technology level and operation time have significant positive effects on land use efficiency of industrial enterprises.