Abstract: To enrich awareness of ichthyotoxic fishes for people, the species, distribution pattern and feeding habits of ichthyotoxic fishes in the Jialing River were studied according to our 8 years investigation results and the existed data. 18 species from 17 genera, 6 families and 4 orders of ichthyotoxic fishes were found in the Jialing River. With the changes of the taxonomy level (from order, family, genus to species), the proportion of ichthyotoxic fishes decreases gradually. Coreius guichenoti and Siniperca chuatsi are absent in the upstream, but the other ichthyotoxic fishes were distributed in whole mainstream of the Jialing River. Analysis of the Jaccard’s similarities indicated that ichthyotoxic fishes were homogeneous distributed in upstream, midstream and downstream in the Jialing River. No significant difference (p>0.05) were found for the distribution pattern of ichthyotoxic fishes by Chi-test. There were two species of ichthyootoxic fishes, one species of ichthyohemotoxic fishes, nine species of ichthyogallotoxic fishes and seven species of acanthotoxic fishes among the 18 ichthyotoxic fish species. The catfish (Silurus asotus) belongs to both ichthyootoxic and acanthotoxic fishes. The ichthyogallotoxic fishes were all from Cyprinidae (Cypriniformes). No ichthyocrinotoxic fishes were found in the Jialing River. Seven trophic guilds were included of the 18 ichthyotoxic fish species, the omnivorous and carnivorous were the dominant trophic guilds, accounting for 38.89% and 33.33% of the ichthyotoxic fish species, respectively. According to our study, we can know that ichthyotoxic fishes are widely distributed in the Jialing River, and there are many species and categories of the ichthyotoxic fishes were found. This study has promoted the understanding of ichthyotoxic fishes and laid the foundation for further study of them in the Jialing River.