Abstract: The pressure on the natural resources and environment by human socio-economic activities, is a crucial factor in threatening regional ecological security.Thus, in order to achieve ecological security, it is important to construct ecological security pattern by considering the constraint of ecological pressure.Taking the Yangtze River Delta of China as the study area, this paper first identified ecological sources based on the Morphological Spatial Pattern Analysis (MSPA) and the landscape probable connectance index method.Then the hot spot regions with ecological pressure were identified by the energy ecological footprint model based on the DMSP/ OLS night-time light data.Finally, both the corridors of ecological sources and that of demand for ecological pressure were extracted by the Minimum Cumulative Resistance (MCR) model.The results showed that: (1) the area of ecological sources was 25 581.47 km², accounting for 24.20% of the total area of the study case, which were primarily distributed in the Taihu lake basin, and the western and eastern Zhejiang.The hot spot regions with ecological pressure were mainly located in highly urbanization, such as Shanghai and Suzhou.There was no spatial overlap between ecological sources and hot spot regions with ecological pressure, thus there was a spatial decoupling to a certain extent between ecological supply and demand.（2) The ecological corridor network consisted of ecological sources corridor and ecological pressure-demand corridor.The total length of ecological sources corridor was 2 459.13 km, presenting a spatial pattern of “two vertical and one horizontal”, and its suitable width was 1 200 m.The total length of the ecological pressure-demand corridor was 1 186.71 km, presenting a spatial cluster with ecological sources as the core.Meanwhile, the suitable width of the ecological pressure-demand corridor with moderate pressure, high pressure and higher pressure was 750, 550 and 100-200 m respectively.（3) The ecological conservation zone, ecological control zone, ecological transition zone, production and living zone, and corridor restoration zone were identified, and their area respectively took up 40.25%, 15.40%, 11.02%, 33.20% and 0.13%.In summary, this study can provide decision support to achieve ecological security and sustainable development in the Yangtze River Delta.