Abstract: Chromophoric dissolved organic matter (CDOM) is an important part of aquatic ecosystem, systematic and comprehensive understanding of CDOM dynamics is critically important for aquatic ecosystem management. In this study, the data of water samples and water quality parameters from 24 sampling sites of Huanggai Lake and inflowing rivers in 4 batches were collected from October to November 2021. Composition characteristics and sources of CDOM in the water of Huanggai Lake were analyzed by three-dimensional fluorescence excitation-emission matrix spectra combined with parallel factor analysis. The results indicated that three fluorescence components were identified by PARAFAC, including two humic-like component and one protein-like component, named C1 (240,310/390)、C2 (265, 350/460) and C3 (220, 280/320). The contribution rates to the total fluorescence intensity of C1, C2 and C3 were 39.58%, 29.34% and 31.08%, respectively. The analysis of the spectral characteristic parameters of fluorescence index (FI), biological index (BIX) and humification index (HIX) showed that there were significant differences in humification degree among different lakes (ANOVA, p<0.05), and the western lake area > the central lake area > the eastern lake area. Correlation analysis showed that C1 was significantly positively correlated with C2 (R= 0.55, P<0.01), C1 in Huanggai Lake was probably derived from the transformation of C2 by microorganisms or the activity of algae. The CDOM in the Huanggai Lake was mainly composed of recent autochthonous components, which were obviously affected by human activities. The main sources may include domestic sewage and farmland leachate using poultry manure in the watershed, microbial biodegradation of residual phytoplankton and terrestrial humic components.