Abstract: In order to promote the Yangtze River conservation, and to achieve sustainable development of circular agriculture, the safety issue of aquaculture wastewater reuse for farmland irrigation was investigated in this research. In March 2022, the concentrations of metal, pesticides, and antibiotics in shrimp/crab and fish aquaculture wastewater, under typical cultivation mode, were observed along the Jiangsu section of the Yangtze River. Nemerowpollution index method and ecological risk index method were adopted to assess the risk levels and feasibility of irrigation. Results showed that Zn was the most abundant heavy metal, and the concentration in shrimp/crab ponds and fish ponds were 19.40 and 15.39 μg·L-1, followed by Ni, As, and Pb. Prometryn was the most abundant pesticide, reaching concentrations of 149.28 ng·L-1, while herbicides and fungicides were the main types of pesticides in aquaculture wastewater. In addition, florfenicol was the most abundant antibiotic, reaching concentrations of 151.40 ng·L-1, while macrolides and amides were the main types of antibiotics in shrimp/crab ponds and fish ponds, respectively. The risk assessment results indicated that heavy metals were below the limit of the ‘Farmland Irrigation Water Quality Standard (GB5084-2021)’, and only Hg in pond S1 had a potential risk, causing the wastewater of shrimp/crab ponds appear to reach the level of slightly-polluted. When considering ecological risks to phytoplankton, both pesticides and antibiotics in fish ponds posed high risks with an average RQ of 4.11 and 36.94, respectively. And in shrimp/crab wastewater, only pesticides posed a high risk towards phytoplankton with an average RQ of 2.72. Hg and metolachlor in shrimp/crab ponds, metolachlor, carbendazim, florfenicol, and sulfamethoxazole in fish ponds were potential risk sources of pollution. However, the contents of metals, pesticides, and antibiotics in aquaculture wastewater were less than the effective concentration of crops. Therefore, aquaculture wastewater could be used for farmland irrigation, and monitoring and treatment of target pollutants are required for long-term application.